activation function purelin Search Results


genomic dna  (Thermo Fisher)
97
Thermo Fisher genomic dna
ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a <t>genomic</t> <t>DNA</t> sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.
Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genomic dna/product/Thermo Fisher
Average 97 stars, based on 1 article reviews
genomic dna - by Bioz Stars, 2026-04
97/100 stars
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purelink genomic dna mini kit  (Thermo Fisher)
99
Thermo Fisher purelink genomic dna mini kit
ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a <t>genomic</t> <t>DNA</t> sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.
Purelink Genomic Dna Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/purelink genomic dna mini kit/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
purelink genomic dna mini kit - by Bioz Stars, 2026-04
99/100 stars
  Buy from Supplier
neural network models  (MathWorks Inc)
96
MathWorks Inc neural network models
ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a <t>genomic</t> <t>DNA</t> sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.
Neural Network Models, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/neural network models/product/MathWorks Inc
Average 96 stars, based on 1 article reviews
neural network models - by Bioz Stars, 2026-04
96/100 stars
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tansig activation function  (MathWorks Inc)
90
MathWorks Inc tansig activation function
ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a <t>genomic</t> <t>DNA</t> sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.
Tansig Activation Function, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tansig activation function/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
tansig activation function - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a genomic DNA sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.

Journal: Molecular Therapy. Nucleic Acids

Article Title: Reactivating Fetal Hemoglobin Expression in Human Adult Erythroblasts Through BCL11A Knockdown Using Targeted Endonucleases

doi: 10.1038/mtna.2016.52

Figure Lengend Snippet: ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a genomic DNA sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.

Article Snippet: Genomic DNA was isolated using the PureLink Genomic DNA mini kit (Life Technologies).

Techniques: TALENs, CRISPR, Binding Assay, Zinc-Fingers, Sequencing, Electroporation, Functional Assay, Activity Assay, Negative Control, Positive Control, Expressing, Plasmid Preparation, Flow Cytometry

ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a genomic DNA sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.

Journal: Molecular Therapy. Nucleic Acids

Article Title: Reactivating Fetal Hemoglobin Expression in Human Adult Erythroblasts Through BCL11A Knockdown Using Targeted Endonucleases

doi: 10.1038/mtna.2016.52

Figure Lengend Snippet: ZFNs, TALENs, and CRISPR/Cas9 nucleases targeting the human BCL11A gene delivered to human hematopoietic cells via plasmids and RNA. ( a ) Schematic of engineered nucleases targeting the BCL11A gene. Top: the human BCL11A gene; bottom: exon 2 with the binding recognition sites of the nucleases (zinc finger nuclease (Z), TALENs (T1, T2), and CRISPR/Cas9 guide sequence targets (g1–4)). The nucleases were delivered through electroporation. ( b ) Representative figure of CEL-1 surveyor assay used to measure the functional activity of the nucleases in the K562 cell line. The type of nuclease used is indicated; negative control (−) was not exposed to a nuclease and the positive control (+) was from a genomic DNA sample previously nuclease-treated and known to have insertions and deletions (indels). The nucleases were delivered at the following concentrations: ZFNs, 2 μg; T1–2, 2 μg; pX330.g1–4, 4 μg. ( c ) Assessment of expression and toxicity after electroporation of GFP expression plasmid and mRNA by flow cytometry (7-AAD) in CD34 + cells, 1 day post-electroporation, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001. CD34 + cells were either suspended in electroporation buffer but not electroporated (untreated), electroporated without added nucleic acid (Mock (E)) or were electroporated with the indicated amounts of GFP plasmid (black bars) or GFP mRNA (gray bars). ( d ) Surveyor assay displaying CD34 + cells electroporated with RNA transcripts of ZFN (5 μg), TALENs (5 μg), and gR.1/Cas9 (5 μg/5 μg). CRISPR, clustered regularly interspaced short palindromic repeat; TALEN, transcriptional activator-like effector nuclease; ZFN, zinc finger nuclease.

Article Snippet: Genomic DNA was isolated using the PureLink Genomic DNA mini kit (Life Technologies).

Techniques: TALENs, CRISPR, Binding Assay, Zinc-Fingers, Sequencing, Electroporation, Functional Assay, Activity Assay, Negative Control, Positive Control, Expressing, Plasmid Preparation, Flow Cytometry